鸡马立克病肝组织肿瘤病变与热休克蛋白转录、表达的相关性研究

doi:10.11843/j.issn.0366-6964.2019.02.020

Abstract

Abstract:

The experiment was conducted to discuss the biological function of HSPs during the progress of tumor induced by the Marek's disease, the relationship between pathological lesion of liver and distribution, expression level of HSPs were studied. The tumor animal model was established using MD virus. Chickens were timely killed post artificial infection and the livers were sampled. Pathological lesion of liver and distribution, transcription and expression level of HSPs were studied during the progress of tumor by the methods of histopathology, immunohistochemistry, FQ RT-PCR and ELISA. Results were as follows:At 14 days, obvious pathological damage appeared in the livers of chickens attracted by MDV, and unequal-sized lymphoid cells infiltration was found among liver cells. HSP60 and HSP90 were mainly located in the cytoplasm of tumor cell, while HSP27 and HSP70 were mainly located in the hepatocyte around the tumor tissues. Within the course of MD, the transcription level of HSP70 and HSP90 mRNA of challenge group were always significantly higher than those of blank control and vaccine control groups. The expression level of HSP90 of challenge group was always significantly higher than those of blank control group in the test process, while the expression level of HSP70 of challenge group was significantly higher than those of blank controrl group at 14 and 21 d, was significantly higher at 28 and 35 d, and no significant difference after 42 d. The distribution among HSP60, HSP90, HSP27, HSP70 and the trend of expression level between HSP90 and HSP70 are completely different during the progress of tumor induced by MDV, which indicates that different HSPs have different biological functions.

CLC Number:

S858.315.3

DUAN Shihao, LU Jianbiao, LI Xuyong, LIU Cheng, ZHANG Shujin, YIN Guozheng, SI Zhenshu, LI Yubao. Relationship between Liver Tissues Pathological Lesion and Expression Level of HSPs in Progress of Marek's Disease[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 415-421.

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Relationship between Liver Tissues Pathological Lesion and Expression Level of HSPs in Progress of Marek's Disease

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